UV-Vis Spectrophotometer

The UV-Vis Spectrophotometer is a high-performance double-beam instrument designed for superior stability, accuracy, and repeatability in analytical measurements. With a 1.5 nm spectral slit width and compliance with European Pharmacopoeia standards, it ensures reliable results across a wide wavelength range of 190–1100 nm. Equipped with photometric, wavelength, and time-scan modes, the DB-20 supports complex analyses like nucleic acid and protein ratio determination. Its large LCD screen, GLP/GMP functions, and flexible standalone or PC-controlled operation make it ideal for laboratory and pharmaceutical use.

Technical features

• The high-performance Halo DB-20 02-beam UVVIS spectrometer simultaneously measures the analysis sample and the reference sample, ensuring stability, accuracy, and repeatability of the measurement results.
• Halo DB-20 02-beam UVVIS spectrometer with 1.5nm spectral slit width; meets European Pharmacopoeia standards
• The Halo DB-20 is equipped with a variety of measurement functions to meet a wide range of analytical requirements:
• Photometric function: efficient quantitative analysis with absorbance (A) or transmittance (T) measurement mode. Select simultaneous measurement at 1 to 6 different wavelengths (determine nucleic acid/protein ratio A260/A280) and set up standard curves up to 20 points with standard solutions of different concentrations.
• Real-time scanning: dynamic analysis over time from 1 minute to > 27 hours.
• Wavelength scanning: full spectrum scanning from 190 to 1100nm with 8 preset speeds from 10nm/min to 3600nm/min. Scan data is displayed as graph or data table, find negative peak/positive peak values.
• The light source uses halogen and D2 lamps, and the wavelength of the lamp can be selected from 325 to 370nm.
• Large 165 x 122mm liquid crystal display (LCD) with adjustable backlight, allowing clear display of measurement parameters, analysis results table, as well as graphs.
• The Halo DB-20 includes a GLP/GMP function that enables the device to evaluate its performance, including wavelength accuracy, repeatability, spectral width, baseline flatness, stability, and contamination. It automatically determines pass/fail status and prints a detailed assessment report.
• Allows to save on the machine 20 operating programs and 10 sets of analysis results data.
• The Halo DB-20 operates independently or connects to a PC for control using UV Detective software.

Specifications:

• Optical system: concave diffraction grating / two beam.
• Wavelength range: 190 ~ 1100 nm
• Spectral slit width: 1.5 nm
• Stray light: ≤ 0.05% (220nm Nal, 340 NaNO2)
• Wavelength accuracy: ± 0.3 nm
• Spectral measurement range:
  • Absorbance (A): – 3 ~ +3
  • Transmittance (%T): 0% ~ 300%T
  • Concentration measurement: 0.000 ~ 9.999
• Wavelength scanning speed: 10, 100, 200, 400, 800, 1200, 2400, 3600 nm/min
• Baseline stability: 0.0003 Abs/hr (500nm, after 2 hrs)
• Contamination: 0.0003 Abs (500nm)
• Light source: Tungsten Halogen and Deuterium lamp
• Light source switching: automatic switching selection from 325 nm ~ 370 nm.
• Detector: Silicon Photodiode
• Display: Back-lit LCD 165(W) x 122(H) mm
• Dimensions (WxDxH) mm: 505 x 705 x 225
• Machine weight: 29 kg
• Power supply: 220V / 50Hz

Why Choose UV-Vis Spectrophotometer?

The UV-Vis Spectrophotometer  offers exceptional precision with its advanced double-beam optical system that simultaneously measures both reference and sample signals for unmatched stability. Its automatic halogen and deuterium light source switching between 325–370 nm ensures continuous, accurate operation. The instrument supports multiple analytical modes, including absorbance, transmittance, and concentration measurements, making it suitable for research, quality control, and pharmaceutical testing. With built-in GLP/GMP validation, it self-assesses wavelength accuracy, baseline stability, and contamination, guaranteeing compliance and reliability. Users benefit from intuitive operation via a large LCD display, 20 programmable methods, and 10 data storage sets. Whether operated independently or through UV Detective software, the Halo DB-20 delivers precision and productivity.